The dodecahedral framework of the bacteriophage phi 6 nucleocapsid is composed of protein P1.
نویسندگان
چکیده
The outer layer of the bacteriophage phi 6 nucleocapsid (NC) was removed by EDTA and reassociated with the core in the presence of Ca2+ or Mg2+. The core was relatively inaccessible to trypsin digestion, was composed of protein P1, and was in the dodecahedral framework reported previously. (H.T. Steely, Jr., and D. Lang, J. Virol. 51:479-483, 1984; Y. Yang and D. Lang, J. Virol. 51:484-488, 1984). The double-stranded RNA genome became RNase sensitive after EDTA treatment of the nucleocapsid.
منابع مشابه
Electron microscopy of bacteriophage phi 6 nucleocapsid: two-dimensional image analysis.
Electron micrographs of negatively stained nucleocapsids isolated from intact, wild-type phi 6 bacteriophage revealed three distinct morphological forms. Two-dimensional analysis of electron micrographs of two of these forms and image averaging of all forms are consistent with a dodecahedral structure embodied in the phi 6 nucleocapsid.
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ورودعنوان ژورنال:
- Journal of virology
دوره 61 8 شماره
صفحات -
تاریخ انتشار 1987